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Good's buffers (also Good buffers) are twenty buffering agents for biochemical and biological research selected and described by Norman Good and colleagues during 1966–1980. [ 1 ] [ 2 ] [ 3 ] Most of the buffers were new zwitterionic compounds prepared and tested by Good and coworkers for the first time, though some ( MES , ADA , BES , Bicine ...
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent.It is one of the twenty Good's buffers.HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in ...
HEPBS (N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid)) is a zwitterionic organic chemical buffering agent; one of Good's buffers. HEPBS and HEPES have very similar structures and properties, HEPBS also having an acidity (pK a) in the physiological range (7.6-9.0 useful range). This makes it possible to use it for cell culture work.
In whole blood (g/cm 3) In plasma or serum (g/cm 3) Water: Solvent 0.81-0.86 0.93-0.95 Acetoacetate: Produced in liver 8-40 × 10 −7: 4-43 × 10 −7: Acetone: product of bodyfat breakdown 3-20 × 10 −6: Acetylcholine: Neurotransmitter of the parasympathetic nervous system: 6.6-8.2 × 10 −8: Adenosine triphosphate: Energy storage total 3. ...
It was developed in the 1960s to provide buffer solutions with pH ranging from 6.15-8.35 for use in various applications. With a pK a of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers ...
In chemistry, a zwitterion (/ ˈ t s v ɪ t ə ˌ r aɪ ə n / TSVIT-ə-ry-ən; from German Zwitter 'hermaphrodite'), also called an inner salt or dipolar ion, [1] is a molecule that contains an equal number of positively and negatively charged functional groups. [2] 1,2-dipolar compounds, such as ylides, are sometimes excluded from the ...
The FDA determined that the data presented in a 2022 color additive petition show that this ingredient causes cancer in male laboratory rats exposed to high levels of FD&C Red No. 3 because of a ...
Carbon dioxide, a by-product of cellular respiration, is dissolved in the blood. From the blood it is taken up by red blood cells and converted to carbonic acid by the carbonate buffer system. Most carbonic acid then dissociates to bicarbonate and hydrogen ions. One of the buffer systems present in the body is the blood plasma buffering system.