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Preclinical imaging is the visualization of living animals for research purposes, [1] such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes.
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy , two-photon excitation microscopy , and multiphoton tomography.
In Fluorescence lifetime and spectral imaging, phasor can be used to visualize the spectra and decay curves. [1] [2] In this method the Fourier transformation of the spectrum or decay curve is calculated and the resulted complex number is plotted on a 2D plot where the X-axis represents the real component and the Y-axis represents the imaginary ...
Fluorescence imaging with one nanometer accuracy (FIONA): utilizes total internal reflection illumination to reduce noise and increase brightness of fluorophores [5] Calcium imaging: technique that utilizes fluorescent molecules called calcium indicators that change in fluorescence when bound to Ca 2+ ions. This is a key part in seeing when ...
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
Biological systems exist as a complex interplay of countless cellular components interacting across four dimensions to produce the phenomenon called life. While it is common to reduce living organisms to non-living samples to accommodate traditional static imaging tools, the further the sample deviates from the native conditions, the more likely the delicate processes in question will exhibit ...
High resolution 99m Tc-MDP mouse scan acquired with a stationary SPECT system: animated image of rotating maximum intensity projections.. Preclinical or small-animal Single Photon Emission Computed Tomography is a radionuclide based molecular imaging modality for small laboratory animals [1] (e.g. mice and rats).
Alternatively the intrinsic fluorescence of a sample (i.e., autofluorescence) can be used. [1] In the life sciences fluorescence microscopy is a powerful tool which allows the specific and sensitive staining of a specimen in order to detect the distribution of proteins or other molecules of interest. As a result, there is a diverse range of ...
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