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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical aperture of the objective lens.
Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. Typical oils used have an index of refraction around 1.515. [2] An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of ...
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...
Some microscopes make use of oil-immersion objectives or water-immersion objectives for greater resolution at high magnification. These are used with index-matching material such as immersion oil or water and a matched cover slip between the objective lens and the sample. The refractive index of the index-matching material is higher than air ...
Live-cell microscopes are generally inverted. To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the ...
Some microscopes use an oil-immersion or water-immersion lens, which can have magnification greater than 100, and numerical aperture greater than 1. These objectives are specially designed for use with refractive index matching oil or water, which must fill the gap between the front element and the object. These lenses give greater resolution ...
Small quantities are used in microscope work as a clearing oil. Today, cedarwood oil is often used for its aromatic properties, especially in aromatherapy; it can also be used to renew the smell of natural cedar furniture. Cedarwood oil is used as an insect repellent, both directly applied to the skin and as an additive to sprays, candles and ...
The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in ... microscope under the oil immersion ...