Search results
Results From The WOW.Com Content Network
These techniques can be combined with microscopy, to map the intensity (confocal microscopy) or the lifetime (fluorescence-lifetime imaging microscopy) of the photoluminescence across a sample (e.g. a semiconducting wafer, or a biological sample that has been marked with fluorescent molecules). Modulated photoluminescence is a specific method ...
Fluorescent minerals emit visible light when exposed to ultraviolet. Fluorescent marine organisms Fluorescent clothes used in black light theater production, Prague. Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation.
This results in a better signal-to-noise ratio, and lowers the detection limit by approximately a factor 10000, [3] when compared to the 180° geometry. Furthermore, the fluorescence can also be measured from the front, which is often done for turbid or opaque samples . [4] The detector can either be single-channeled or multichanneled.
Photoluminescence, a result of the absorption of photons Fluorescence, traditionally defined as the emission of light that ends immediately after the source of excitation is removed. As the definition does not fully describe the phenomenon, quantum mechanics is employed where it is defined as there is no change in spin multiplicity from the ...
Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength. Unlike fluorescence, a phosphorescent material does not immediately reemit the radiation it absorbs.
The quest for fluorescent probes with a high specificity that also allow live imaging of plant cells is ongoing. [7] There are many fluorescent molecules called fluorophores or fluorochromes such as fluorescein, Alexa Fluors, or DyLight 488, which can be chemically linked to a different molecule which binds the target of interest within the sample.
Piezospectroscopy can be used on any material that exhibits fluorescence, but is almost exclusively used on samples containing alumina because of the presence of chromium ions, either as part of the composition or as an impurity, that greatly increase the fluorescent response.
Multicolor fluorescence image of living HeLa cells. Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy.