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Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
Most of the dyes commonly used in microscopy are available as BSC-certified stains. This means that samples of the manufacturer's batch have been tested by an independent body, the Biological Stain Commission ( BSC ), and found to meet or exceed certain standards of purity, dye content and performance in staining techniques ensuring more ...
In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. [1]: 235 [2] [3] [4] Perls Prussian Blue derives its name from the German pathologist Max Perls (1843–1881), who described the technique in 1867. [2]
The most commonly used stain in histology is a combination of hematoxylin and eosin (often abbreviated H&E). Hematoxylin is used to stain nuclei blue, while eosin stains the cytoplasm and the extracellular connective tissue matrix of most cells pink. There are hundreds of various other techniques which have been used to selectively stain cells.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Methods for mordant application depend on the desired stain and tissues under study; pre-, meta- and post-mordanting techniques are used as required. The most commonly used stain used in diagnostic histology of animal tissues is Harris' haematoxylin as part of a haematoxylin and eosin (H&E) stain.
Papanicolaou stain (also Papanicolaou's stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. [ 1 ] [ 2 ] [ 3 ] The Papanicolaou stain is one of the most widely used stains in cytology , [ 1 ] where it is used to aid pathologists in making a diagnosis.