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Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required.
Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Methods for mordant application depend on the desired stain and tissues under study; pre-, meta- and post-mordanting techniques are used as required. The most commonly used stain used in diagnostic histology of animal tissues is Harris' haematoxylin as part of a haematoxylin and eosin (H&E) stain.
Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Lillie's trichrome is a combination of dyes used in histology.. It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [1]
In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. [ 1 ] : 235 [ 2 ] [ 3 ] [ 4 ] Perls Prussian Blue derives its name from the German pathologist Max Perls (1843–1881), who described the technique in 1867. [ 2 ]
It is used to stain collagen. If blue is preferred to green, methyl blue or water blue can be substituted. Standard applications : Masson's trichrome staining is widely used to study muscular pathologies ( muscular dystrophy ), cardiac pathologies ( infarct ), hepatic pathologies ( cirrhosis ) or kidney pathologies (glomerular fibrosis).