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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
The CRFs in thin layer chromatography characterize the equal-spreading of the spots. The ideal case, when the RF of the spots are uniformly distributed in <0,1> range (for example 0.25,0.5 and 0.75 for three solutes) should be characterized as the best situation possible.
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
High-performance thin-layer chromatography (HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds. [1] This TLC-based analytical technique enhances compound resolution for quantitative analysis.
These amino acids are then separated using 2-dimensional thin layer chromatography, along with amino acid standards for the three amino acids that are phosphorylated in eukaryotes: serine, threonine, and tyrosine.
Chiral thin-layer chromatography is a variant of liquid chromatography that is employed for the separation of enantiomers. It is necessary to use either a chiral stationary phase or; a chiral additive in the mobile phase.
GCSE Bitesize was launched in January 1998, covering seven subjects. For each subject, a one- or two-hour long TV programme would be broadcast overnight in the BBC Learning Zone block, and supporting material was available in books and on the BBC website. At the time, only around 9% of UK households had access to the internet at home.
Thin layer extraction is a time-periodic reactive liquid extraction process that provides excellent mass transfer while maintaining phase separation. [1] It is performed via a periodic batch production process that controls the time of each chemical reaction .