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Methylated DNA immunoprecipitation (MeDIP or mDIP) is a large-scale (chromosome- or genome-wide) purification technique in molecular biology that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5-methylcytosine (5mC).
MeRIPseq [1] (or MeRIP-seq) stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications, developed by Kate Meyer et al. while working in the laboratory of Sammie Jaffrey at Cornell University Graduate School of Medical Sciences.
Methylated DNA immunoprecipitation (MeDIP), analogous to chromatin immunoprecipitation, immunoprecipitation is used to isolate methylated DNA fragments for input into DNA detection methods such as DNA microarrays (MeDIP-chip) or DNA sequencing (MeDIP-seq). Pyrosequencing of bisulfite treated DNA. This is the sequencing of an amplicon made by a ...
iCLIP [1] [2] [3] (individual-nucleotide resolution crossLinking and immunoprecipitation) is a variant of the original CLIP method used for identifying protein-RNA interactions, [4] which uses UV light to covalently bind proteins and RNA molecules to identify RNA binding sites of proteins.
Bayesian tool for methylation analysis, also known as BATMAN, is a statistical tool for analysing methylated DNA immunoprecipitation (MeDIP) profiles. It can be applied to large datasets generated using either oligonucleotide arrays (MeDIP-chip) or next-generation sequencing (MeDIP-seq), providing a quantitative estimation of absolute methylation state in a region of interest.
These two techniques, called m6A-seq and MeRIP-seq (m6A-specific methylated RNA immunoprecipitation), are also the first methods to allow for any type of RNA modification sequencing. These methods were able to detect 10,000 m6A peaks in the mammalian transcriptome; the peaks were found to be enriched in 3’UTR regions, near STOP codons, and ...
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.
In chemistry, coprecipitation (CPT) or co-precipitation is the carrying down by a precipitate of substances normally soluble under the conditions employed. [1] Analogously, in medicine, coprecipitation (referred to as immunoprecipitation) is specifically "an assay designed to purify a single antigen from a complex mixture using a specific antibody attached to a beaded support".