Search results
Results From The WOW.Com Content Network
The column is free from initial stress. The weight of the column is neglected. The column is initially straight (no eccentricity of the axial load). Pin joints are friction-less (no moment constraint) and fixed ends are rigid (no rotation deflection). The cross-section of the column is uniform throughout its length.
where L is the column length and N the number of theoretical plates. [5] The relation between plate number and peak width at the base is given by
To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied. Depending on the application material- and size-requirements may change. Gas chromatography (GC): Older columns were made of glass or metal packed with particles of a solid stationary phase. More recently ...
The slenderness ratio is an indicator of the specimen's resistance to bending and buckling, due to its length and cross section. If the slenderness ratio is less than the critical slenderness ratio, the column is considered to be a short column. In these cases, the Johnson parabola is more applicable than the Euler formula. [5]
The effective length is calculated from the actual length of the member considering the rotational and relative translational boundary conditions at the ends. Slenderness captures the influence on buckling of all the geometric aspects of the column, namely its length, area, and second moment of area.
The size will depend on the amount of compound being isolated. The base of the tube contains a filter, either a cotton or glass wool plug, or glass frit to hold the solid phase in place. A solvent reservoir may be attached at the top of the column. Two methods are generally used to prepare a column: the dry method and the wet method.
The column space of a matrix is the image or range of the corresponding matrix transformation. Let be a field. The column space of an m × n matrix with components from is a linear subspace of the m-space. The dimension of the column space is called the rank of the matrix and is at most min(m, n). [1]
A size exclusion column. Each size exclusion column has a range of molecular weights that can be separated. The exclusion limit defines the molecular weight at the upper end of the column 'working' range and is where molecules are too large to get trapped in the stationary phase.