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MCF-7 cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2] The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer ...
To accomplish the E-SCREEN assay the cells are trypsinized and plated in well culture plates. Cells are allowed to attach for 24 h, and the 4 seeding medium is then removed and replaced with the experimental culture medium (phenol red free DMEM with charcoal dextran treated fetal bovine serum -steroid-free-). MCF-7 cell. A tissue culture hood
Integrated discrete Multiple Organ Culture (IdMOC) is an in vitro, cell culture based experimental model for the study of intercellular communication. In conventional in vitro systems, each cell type is studied in isolation ignoring critical interactions between organs or cell types. IdMOC technology is based on the concept that multiple organs ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Immortalised cell lines are widely used as a simple model for more complex biological systems – for example, for the analysis of the biochemistry and cell biology of mammalian (including human) cells. [2] The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture.
Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.
Thymidine 0.7 mg Glycine 7.51 mg Cyanocobalamin 1.3 mg L-Serine 10.5 mg Sodium pyruvate 110 mg L-Threonine 3.57 mg Lipoic acid 0.2 mg L-Aspartic acid 13.3 mg CaCl 2 44 mg L-Glutamic acid 14.7 mg MgSO 4 ·7H 2 O 153 mg L-Asparagine 15 mg Glucose 1.1 g L-Glutamine 146.2 mg NaCl 7.4 g L-Isoleucine 2.6 mg KCl 285 mg L-Leucine 13.1 mg
Pages in category "Cell culture media" The following 36 pages are in this category, out of 36 total. This list may not reflect recent changes. A. Antibiotic-Antimycotic;
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