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It was used by the team to characterize several protein complexes (Rigaut et al., 1999, [2] Caspary et al. 1999, [4] Bouveret et al., 2000, [5] Puig et al., 2001 [3]). The first large-scale application of this technique was in 2002, in which the research team worked in collaboration with scientists of the proteomics company Cellzome to develop ...
TAP-mediated peptide transport is a multistep process. The peptide-binding pocket is formed by TAP-1 and TAP-2. Association with TAP is an ATP-independent event, ‘in a fast bimolecular association step, peptide binds to TAP, followed by a slow isomerisation of the TAP complex’. [5]
Biochemistry, or biological chemistry, is the study of chemical processes within and relating to living organisms. [1] A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology, and metabolism. Over the last decades of the 20th century, biochemistry has become successful at ...
Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological systems.
Lab notebook with the complete record of the experiments underlying a published paper. [1] Chemistry stencils that used to be used for drawing equipment in lab notebooks. A laboratory notebook ( colloq. lab notebook or lab book ) is a primary record of research .
The United States has a documented shortage of working laboratory professionals. For example, as of 2016 vacancy rates for Medical Laboratory Scientists ranged from 5% to 9% for various departments. The decline is primarily due to retirements, and to at-capacity educational programs that cannot expand which limits the number of new graduates.
Laboratory methods and techniques, as used in fields like biology, biochemistry, biophysics, chemistry, molecular biology, etc. Wikimedia Commons has media related to Laboratory techniques . Contents
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.