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Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
The propagation of shoots or nodal segments is usually performed in four stages for mass production of plantlets through in vitro vegetative multiplication but organogenesis is a standard method of micropropagation that involves tissue regeneration of adventitious organs or axillary buds directly or indirectly from the explants.
This technique is also called micropropagation. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.
Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake".
Pages in category "Micropropagation" The following 2 pages are in this category, out of 2 total. This list may not reflect recent changes. ...
These primary cells can then be further expanded and transferred into fresh dishes through micropropagation. Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, [ 3 ] or ...
Micropropagation of plants is the process of using small samples of plants called explants and causing them to undergo growth of differentiated or undifferentiated cells. In connection with cytokinins like kinetin , auxins like IBA can be used to cause the formation of masses of undifferentiated cells called callus .
Micropropagation involves growing plants from very small amounts of source tissue, sometimes even a single cell, under sterile conditions using artificial techniques to induce growth from mitochondrial relief systems. The purpose of micropropagation is often to produce a large number of genetically identical offspring in the manner of Shannon ...