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A semen analysis (plural: semen analyses), also called seminogram or spermiogram, evaluates certain characteristics of a male's semen and the sperm contained therein. [1] [2] [3] It is done to help evaluate male fertility, whether for those seeking pregnancy or verifying the success of vasectomy. Depending on the measurement method, just a few ...
When performing cryopreservation of semen, it is the sperm quality after reviving the sample that is of importance, because many sperm cells die in the process. To be of use in assisted reproductive technology, the sample should after thawing have more than 5 million motile sperm cells per ml with a good grade of mobility. If the grade of ...
Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]
Semen sample obtaining is the first step in spermiogram. The optimal sexual abstinence for semen sample obtaining is of 2–7 days. The first way to obtain the semen sample is through masturbation, and the best place to obtain it is in the same clinic, as this way temperature changes during transport can be avoided, which can be lethal for some ...
Confirmatory tests for semen include the christmas tree stain and the p30/PSA RSID kit. For the christmas tree stain, the sample is extracted with sterile water in order to make a wet mount on a microscope slide. The sample is then heat-fixed to the slide and stained with nuclear fast red for 15 minutes, then rinsed with deionized water. [8]
Once the fastest sperm have been isolated, before using them for artificial insemination or in vitro fertilization, it is important to confirm the absence of HIV virus in the sample. The sample obtained after washing is analysed, usually using the PCR technique, to check that there is no viral particle. If the result is negative, i.e. there is ...
Methods of semen collection from humans include: Masturbation, directing the sample into a clean cup. [6] This is the most common way to collect a semen sample. [6] Sexual intercourse using a special type of condom known as a collection condom. [6] Collection condoms are made from silicone or polyurethane, as latex is somewhat harmful to sperm ...
Here are links to possibly useful sources of information about Semen analysis. PubMed provides review articles from the past five years (limit to free review articles) The TRIP database provides clinical publications about evidence-based medicine. Other potential sources include: Centre for Reviews and Dissemination and CDC