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Many biotechnology applications utilize mutated plasmids that replicate to high copy number. For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high copy number cloning vectors (>100 copies/cell) have been derived by mutagenesis, such as the well known pUC series. [1]
Generally, each molecule of plasmid diffuses randomly, so the probability of having a plasmid-less daughter cell is 2 1−N, where N is the number of copies. For instance, if there are 2 copies of a plasmid in a cell, there is 50% chance of having one plasmid-less daughter cell. However, high-copy number plasmids have a cost for the hosting cell.
The pUC plasmids are all circular double stranded DNA about 2700 base pairs in length. [3] ... pUC19 is a high copy number plasmid. [3]
Many plasmids have high copy numbers, for example, pUC19 has a copy number of 500-700 copies per cell, [6] and high copy number is useful as it produces greater yield of recombinant plasmid for subsequent manipulation. However low-copy-number plasmids may be preferably used in certain circumstances, for example, when the protein from the cloned ...
Larger plasmids tend to have lower copy numbers. [15] Low-copy-number plasmids that exist only as one or a few copies in each bacterium are, upon cell division, in danger of being lost in one of the segregating bacteria. Such single-copy plasmids have systems that attempt to actively distribute a copy to both daughter cells.
This ensures that at high copy numbers, ... ColE1 has a copy number of 25-30 according to its original publication. [2] References This page was ...