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Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones. [1] It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia. [2] [3] [4] It has utility for research and clinical care. It was created by, and is named after, Raymond Sabouraud in 1892.
Colonies on Potato dextrose agar (PDA) after 3 days incubation at 30 °C (86 °F) are white in colour and reach 3 mm in diameter. [4] Cultures developed on Sabouraud dextrose agar (SDA) are similar in appearance although they grow more slowly on this medium. [4]
The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
Sabouraud agar is used to culture fungi and has a low pH that inhibits the growth of most bacteria; it also contains the antibiotic gentamicin to specifically inhibit the growth of Gram-negative bacteria. Hay infusion agar is specific for the culturing of slime moulds (which are not fungi). Potato dextrose agar is used to culture certain types ...
DTM plate on the right showing color change after two days' growth; Microsporum canis Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. [1] [2] It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator.
Rhodotorula mucilaginosa cells, Methylene blue stain, magnification 400x. Rhodotorula is a genus of fungi in the class Microbotryomycetes.Most species are known in their yeast states which produce orange to red colonies when grown on Sabouraud's dextrose agar (SDA).
Zygomycetes grow well on most standard fungal culture medium such as Sabouraud dextrose agar. They can also grow on both selective and non-selective media. Minimal media, supplementary media and induction media can also be used.
3.2 Incubation. 3.3 Identification. ... To isolate fungi, Sabouraud agar can be used. ... and for different lengths of time, ...