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The sample throughput for 3' mRNA-seq library preparation differs per method but can allow up to 384 samples to be processed in plates, with options for automation. [2] [12] For methods where samples are pooled early in the workflow, consumable use and cost are further reduced. For instance, BRB-seq is up to 25 times cheaper than Illumina ...
Sequencing technology platforms commonly used for RNA-Seq [77] [78] Platform Commercial release Typical read length Maximum throughput per run Single read accuracy RNA-Seq runs deposited in the NCBI SRA (Oct 2016) [79] 454 Life Sciences: 2005 700 bp 0.7 Gbp 99.9% 3548 Illumina: 2006 50–300 bp 900 Gbp 99.9% 362903 SOLiD: 2008 50 bp 320 Gbp 99. ...
RNA-Seq [1] [2] [3] is a technique [4] that allows transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools developed to support the different steps of the process.
RNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome.
RNA-SSD is publicly available under the name of RNA Designer at the RNASoft web page: Web Server [197] INFO-RNA: INFO-RNA uses a dynamic programming approach to generate an energy optimized starting sequence that is subsequently further improved by a stochastic local search that uses an effective neighbor selection method. Web Server Source ...
It can map Illumina and SOLiD reads. Unlike most mapping programs, speed increases for longer read lengths. Yes Free, GPL [49] PRIMEX Indexes the genome with a k-mer lookup table with full sensitivity up to an adjustable number of mismatches. It is best for mapping 15-60 bp sequences to a genome. No No Yes No, multiple processes per search 2003