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Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Chocolate agar (CHOC) or chocolate blood agar (CBA) is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. [ 1 ] [ 2 ] [ 3 ] It is a variant of the blood agar plate , containing red blood cells that have been lysed by slowly heating to 80°C.
Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a Mueller–Hinton agar with 5% chocolate sheep blood and antibiotics. It is used for culturing and primarily isolating pathogenic Neisseria bacteria , including Neisseria gonorrhoeae and Neisseria meningitidis , as the medium inhibits the growth of most other microorganisms.
Agar plates Organisms are placed or streaked onto petri dishes Provides a solid surface for stationary growth, compact and stackable Agar based dipsticks Essentially miniature agar plates in the form of dipsticks Diagnostic purposes, can be used anywhere, cost effective, easy to use Selective and differential media
Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus, [1] formerly part of the "group D streptococci" (enterococci were reclassified in their own genus in 1984).
Streptolysin O is an oxygen-sensitive cytotoxin secreted by most Group A streptococcus (GAS) and Streptococcus dysgalactiae; it interacts with cholesterol in the membrane of eukaryotic cells (mainly red and white blood cells, macrophages, and platelets), usually resulting in β-hemolysis under the surface of blood agar. Streptolysin S is an ...
When plated on sheep or bovine blood agar, S. pseudintermedius displays incomplete ß-hemolysis. [20] [8] Colonies of S. pseudintermedius on sheep agar are described as medium in size and non-pigmented or grey-white.