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TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR.The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, [1] and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher Scientific) for research applications.
List of free analog and digital electronic circuit simulators, available for Windows, macOS, Linux, and comparing against UC Berkeley SPICE.The following table is split into two groups based on whether it has a graphical visual interface or not.
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by master's student Alice Chien et al. in 1976. [1]
diagrams.net (previously draw.io [2] [3]) is a cross-platform graph drawing software application developed in HTML5 and JavaScript. [4] Its interface can be used to create diagrams such as flowcharts , wireframes , UML diagrams, organizational charts , and network diagrams .
Fluorescent reporter probes detect only the DNA containing the sequence complementary to the probe; therefore, use of the reporter probe significantly increases specificity, and enables performing the technique even in the presence of other dsDNA. Using different-coloured labels, fluorescent probes can be used in multiplex assays for monitoring ...
Since the TaqMan assay is based on PCR, it is relatively simple to implement. The TaqMan assay can be multiplexed by combining the detection of up to seven SNPs in one reaction. However, since each SNP requires a distinct probe, the TaqMan assay is limited by the how close the SNPs can be situated. The scale of the assay can be drastically ...
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