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The Kjeldahl method or Kjeldahl digestion (Danish pronunciation: [ˈkʰelˌtɛˀl]) in analytical chemistry is a method for the quantitative determination of a sample's organic nitrogen plus ammonia/ammonium (NH 3 /NH 4 +). Without modification, other forms of inorganic nitrogen, for instance nitrate, are not included in
His laboratory technique for nitrogen and protein analysis is still the universally accepted method for this analysis. Although other methods claim to be faster and more efficient, none can cope with the variety of sizes or conditions of samples than Johan Kjeldahl's original method. Kjeldahl equipment is used extensively all over the world.
The AutoAnalyzer is an automated analyzer using a flow technique called continuous flow analysis (CFA), or more correctly segmented flow analysis (SFA) first made by the Technicon Corporation. The instrument was invented in 1957 by Leonard Skeggs, PhD and commercialized by Jack Whitehead's Technicon Corporation.
The measured signal from the thermal conductivity detector for the unknown sample can then be converted into a nitrogen content. As with the Kjeldahl method, conversion of the concentration of nitrogen in a sample to the crude protein content is performed using conversion factors which depend on the particular amino acid sequence of the ...
Over time, this became a separate branch of analytical chemistry called instrumental analysis. Because of the high volume of wet chemistry that must be done in today's society and new quality control requirements, many wet chemistry methods have been automated and computerized for streamlined analysis. The manual performance of wet chemistry ...
For example, if a gas mixture such as air is passed under pressure through a vessel containing an adsorbent bed of zeolite that attracts nitrogen more strongly than oxygen, a fraction of nitrogen will stay in the bed, and the gas exiting the vessel will be richer in oxygen than the mixture entering. When the bed reaches the limit of its ...
Flow injection analysis (FIA) was first described by Ruzicka and Hansen in Denmark in 1974 and Stewart and coworkers in United States in 1979. FIA is a popular, simple, rapid, and versatile technique which is a well-established position in modern analytical chemistry, and widespread application in quantitative chemical analysis.
Diagram of quenched flow apparatus for following reactions with half times of a few milliseconds. The stopped-flow method relies on the presence of spectroscopic properties to monitor reactions in real time. When such properties are unavailable, quenched-flow provides an alternative by using conventional chemical analysis. [8]