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  2. Minichromosome - Wikipedia

    en.wikipedia.org/wiki/Minichromosome

    Minichromosomes can be either linear or circular pieces of DNA. [3] By minimizing the amount of unnecessary genetic information on the chromosome and including the basic components necessary for DNA replication (centromere, telomeres, and replication sequences), molecular biologists aim to construct a chromosomal platform which can be utilized to insert or present new genes into a host cell.

  3. HEK 293 cells - Wikipedia

    en.wikipedia.org/wiki/HEK_293_cells

    Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [ 1 ] [ 2 ] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection .

  4. Minichromosome maintenance - Wikipedia

    en.wikipedia.org/wiki/Minichromosome_Maintenance

    The minichromosome maintenance protein complex (MCM) is a DNA helicase essential for genomic DNA replication. Eukaryotic MCM consists of six gene products, Mcm2–7, which form a heterohexamer.

  5. Engineering biology - Wikipedia

    en.wikipedia.org/wiki/Engineering_biology

    Engineering biology is the set of methods for designing, building, and testing engineered biological systems which have been used to manipulate information, construct materials, process chemicals, produce energy, provide food, and help maintain or enhance human health and environment.

  6. Reverse genetics - Wikipedia

    en.wikipedia.org/wiki/Reverse_genetics

    Diagram illustrating the development process of avian flu vaccine by reverse genetics techniques. Reverse genetics is a method in molecular genetics that is used to help understand the function(s) of a gene by analysing the phenotypic effects caused by genetically engineering specific nucleic acid sequences within the gene.

  7. Kidney development - Wikipedia

    en.wikipedia.org/wiki/Kidney_development

    The development of the kidney proceeds through a series of successive phases, each marked by the development of a more advanced kidney: the archinephros, pronephros, mesonephros, and metanephros. [1] The pronephros is the most immature form of kidney, while the metanephros is most developed. The metanephros persists as the definitive adult kidney.

  8. Organ culture - Wikipedia

    en.wikipedia.org/wiki/Organ_culture

    Organ culture is the cultivation of either whole organs or parts of organs in vitro. [1] It is a development from tissue culture methods of research, as the use of the actual in vitro organ itself allows for more accurate modelling of the functions of an organ in various states and conditions.

  9. Renal physiology - Wikipedia

    en.wikipedia.org/wiki/Renal_physiology

    Changes in renin ultimately alter the output of this system, principally the hormones angiotensin II and aldosterone. Each hormone acts via multiple mechanisms, but both increase the kidney's absorption of sodium chloride, thereby expanding the extracellular fluid compartment and raising blood pressure. When renin levels are elevated, the ...

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