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The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression. [3] [2] T7 has been used as a model in synthetic biology. Chan et al. (2005) "refactored" the genome of T7, replacing approximately 12 kbp of its genome with engineered DNA. [15]
In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different polymerases.
The T7 expression system is used in the field of microbiology to clone recombinant DNA using strains of E. coli. [1] It is the most popular system for expressing recombinant proteins in E. coli. [2] By 2021, this system had been described in over 220,000 research publications. [3]
T7 phage group was renamed to T7-like phages in the sixth report in 1995. In 1998, the whole family was moved into the newly created order Caudovirales , and the genus was renamed again in the seventh report in 1999 to T7-like viruses . 2009 saw the genus moved into the newly created subfamily Autographivirinae , and it was renamed again in ...
Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated.
Deutsch: Schematische Darstellung der Struktur des T7-Phagen. Italiano: Rappresentazione schematica della struttura del fago T7. Español: Representación esquemática de la estructura del fago T7.
Podoviridae was a family of bacteriophage in the order Caudovirales often associated with T-7 like phages. [1] The family and order Caudovirales have now been abolished, with the term podovirus now used to refer to the morphology of viruses in this former family. [2]
The resulting thioredoxin-gp5 complex increases the affinity of T7 polymerase for the primer terminus by ~80-fold and acts processively around 800 nucleotide incorporation steps. [ 12 ] The mechanism adopted by T7 polymerase to achieve its processivity differs from many other polymerases in that it does not rely on a DNA clamp or a clamp loader.