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MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. [1] Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color.
Pseudomonas aeruginosa is a common encapsulated, ... In mixed cultures, it can be isolated as clear colonies on MacConkey agar (as it does not ferment lactose) ...
MacConkey agar is a selective and differential medium used to differentiate between gram-negative bacteria while inhibiting the growth of gram-positive bacteria. Adding bile salts and crystal violet to the agar inhibits the growth of most gram-positive bacteria, making MacConkey agar selective.
Cetrimide also enhances the production of Pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic blue-green and yellow-green colour, respectively. [3] [4] Cetrimide agar is widely used in the examination of cosmetics, pharmaceuticals and clinical specimens to test for the presence of Pseudomonas aeruginosa. [1]
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
VRDBA, VRDB Agar, or Violet Red Bile Dextrose Agar, is a microbiological growth medium. [1] It can be used in agar plates to monitor or assess bacterial growth in the laboratory, particularly the growth of Enterobacteriaceae , for which it is selective.
It appears as a mucoid lactose fermenter on MacConkey agar. Although found in the normal flora of the mouth, skin, and intestines, [1] it can cause destructive changes to human and animal lungs if aspirated, specifically to the alveoli, resulting in bloody, brownish or yellow colored jelly-like sputum.