Ads
related to: affinity chromatography sources
Search results
Results From The WOW.Com Content Network
Affinity chromatography is a method of separating a biomolecule from a mixture, ... Inorganic sources are moronic acid, metal chelates and triazine dyes. [7]
Agarose-lectin column chromatography, lectin affinity chromatography: To purify glycoproteins or glycopeptides that bind the particular lectin used. Lectin affinity electrophoresis: Resultant shifts in electrophoretic migration help distinguish and characterize glycoforms, i.e. variants of a glycoprotein differing in carbohydrate.
The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography.The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys).
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [1] as well as in research and development. When purifying antibodies, protein A is used as affinity matrix ...
It is one of the most specific tags [2] and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells.
Proteins can coordinate metal ions on their surface and it is possible to separate proteins using chromatography by making use of the difference in their affinity to metal ions. This is termed as immobilized metal ion affinity chromatography (IMAC), as originally introduced in 1975 under the name metal chelate affinity chromatography. [3]