Ads
related to: immobilized phase chromatography
Search results
Results From The WOW.Com Content Network
Affinity chromatography has the advantage of specific binding interactions between the analyte of interest (normally dissolved in the mobile phase), and a binding partner or ligand (immobilized on the stationary phase).
Immobilized phase – a stationary phase that is immobilized on the support particles, or on the inner wall of the column tubing. Mobile phase – the phase that moves in a definite direction. It may be a liquid (LC and capillary electrochromatography , CEC), a gas (GC), or a supercritical fluid (supercritical-fluid chromatography, SFC).
The chiral stationary phase (CSP) is made of a support, usually silica based, on which a chiral reagent or a macromolecule with numerous chiral centers is bonded or immobilized. [3] The chiral stationary phase can be prepared by attaching a chiral compound to the surface of an achiral support such as silica gel.
Centrifugal partition chromatography is a special chromatographic technique where both stationary and mobile phase are liquid, and the stationary phase is immobilized by a strong centrifugal force. Centrifugal partition chromatography consists of a series-connected network of extraction cells, which operates as elemental extractors, and the ...
Affinity chromatography is performed following one of two basic formats: ligand immobilization or target immobilization. Under the ligand immobilization format, a ligand of interest - often a drug lead - is immobilized within a chromatography column and acts as the stationary phase.
High performance affinity chromatography (HPAC) [33] works by passing a sample solution through a column packed with a stationary phase that contains an immobilized biologically active ligand. The ligand is in fact a substrate that has a specific binding affinity for the target molecule in the sample solution.
Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography, but using dyes to apply on a support matrix of a column as the stationary phase that will allow a range of proteins with similar active sites to bind to, refers to as pseudo-affinity.
A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...