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RNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome.
The word transcriptome is a portmanteau of the words transcript and genome.It appeared along with other neologisms formed using the suffixes -ome and -omics to denote all studies conducted on a genome-wide scale in the fields of life sciences and technology.
The fragmentation method is a key aspect of sequencing library construction. Fragmentation may be achieved by chemical hydrolysis, nebulisation, sonication, or reverse transcription with chain-terminating nucleotides. [67] Alternatively, fragmentation and cDNA tagging may be done simultaneously by using transposase enzymes. [68] During ...
CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is a method for performing RNA sequencing along with gaining quantitative and qualitative information on surface proteins with available antibodies on a single cell level. [1] So far, the method has been demonstrated to work with only a few proteins per cell.
Spatial transcriptomics is a method that captures positional context of transcriptional activity within intact tissue [1].The historical precursor to spatial transcriptomics is in situ hybridization [2], where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.
Originally, transcriptome-wide RNA abundance could only be assessed using methods such as DNA microarrays or serial analysis of gene expression (SAGE). [4] [5] These methods are prohibitive in differing regards; microarrays, while cheap, provide inconsistent results [6] and SAGE is based on sanger sequencing, which provides limited throughput ...
G&T-seq (short for single cell genome and transcriptome sequencing) is a novel form of single cell sequencing technique allowing one to simultaneously obtain both transcriptomic and genomic data from single cells, allowing for direct comparison of gene expression data to its corresponding genomic data in the same cell...
In molecular phylogenetics, relationships among individuals are determined using character traits, such as DNA, RNA or protein, which may be obtained using a variety of sequencing technologies. High-throughput next-generation sequencing has become a popular technique in transcriptomics, which represent a