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An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. [1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding ...
The two most commonly used inducible expression systems for research of eukaryote cell biology are named Tet-Off and Tet-On. [3] The Tet-Off system for controlling expression of genes of interest in mammalian cells was developed by Professors Hermann Bujard [] and Manfred Gossen at the University of Heidelberg and first published in 1992.
Monoallelic gene expression (MAE) is the phenomenon of the gene expression, when only one of the two gene copies is actively expressed (transcribed), while the other is silent. [ 1 ] [ 2 ] [ 3 ] Diploid organisms bear two homologous copies of each chromosome (one from each parent), a gene can be expressed from both chromosomes (biallelic ...
Regulation of gene expression, or gene regulation, [1] includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products (protein or RNA). Sophisticated programs of gene expression are widely observed in biology, for example to trigger developmental pathways, respond to environmental ...
An inducible gene is a gene whose expression is either responsive to environmental change or dependent on the position in the cell cycle. Any step of gene expression may be modulated, from the DNA-RNA transcription step to post-translational modification of a protein. The stability of the final gene product, whether it is RNA or protein, also ...
Cre-Lox recombination is a special type of site-specific recombination developed by Dr. Brian Sauer and patented by DuPont that operated in both mitotic and non-mitotic cells, and was initially used in activating gene expression in mammalian cell lines.
To control gene expression spatially inkjet printers are under development for printing ligands on gel culture. [1] Other popular method involves use of light to control gene expression in spatiotemporal fashion. Since light can also be controlled easily in space, time and degree, several methods of controlling gene expression at DNA and RNA ...
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. Such genes are called reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because ...