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Spin column-based nucleic acid purification. Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.
Plasmid miniprep. 0.8% agarose gel ethidium bromide -stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1][2] Many methods have been developed to purify ...
QIAGEN N.V. is a German-founded multinational provider of sample and assay technologies for molecular diagnostics, applied testing, academic research, and pharmaceutical research. The company operates in more than 35 offices in over 25 countries. QIAGEN N.V., the global corporate headquarter of the QIAGEN group, is located in Venlo, The ...
DNA extraction. The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other ...
A mobility shift assay is electrophoretic separation of a protein–DNA or protein–RNA mixture on a polyacrylamide or agarose gel for a short period (about 1.5-2 hr for a 15- to 20-cm gel). [4] The speed at which different molecules (and combinations thereof) move through the gel is determined by their size and charge, and to a lesser extent ...
The highest DNA adsorption efficiencies occur in the presence of buffer solution with a pH at or below the pKa of the surface silanol groups. The mechanism behind DNA adsorption onto silica is not fully understood; one possible explanation involves reduction of the silica surface's negative charge due to the high ionic strength of the buffer.
In molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease. [2][3][4] The enzyme was discovered in 1974 in extracts of the fungus Parengyodontium album (formerly Engyodontium album or ...
Synchronous coefficient of drag alteration ( SCODA) is a biotechnology method for purifying, separating and/or concentrating bio-molecules. SCODA has the ability to separate molecules whose mobility (or drag) can be altered in sync with a driving field. This technique has been primarily used for concentrating and purifying DNA, where DNA ...