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The first impedance-based flow cytometry device, using the Coulter principle, was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter.Mack Fulwyler was the inventor of the forerunner to today's flow cytometers – particularly the cell sorter. [6]
Moreover, flow cytometry cell-sorters are complex instruments that are generally used only by well-trained staff in flow cytometry facilities or well-equipped laboratories and, since they are normally large in size, it is not always possible to place them inside a biological safety cabinet. Therefore, it is not always possible to ensure sample ...
These results from each cell are cumulated and assigned in a calibrated multi-channel analyser with over 500,000 channels. So, for the CASY technology, as the cell flow cytometry, it can present data of each cell as a cell size distribution graph, which has 2 variables, the change in cell volume and that in cell viability. The materials passing ...
Cell populations detected by flow cytometry are often described as having approximately log-normal expression. [15] As such, they have traditionally been transformed to a logarithmic scale. In early cytometers, this was often accomplished even before data acquisition by use of a log amplifier. On modern instruments, data is usually stored in ...
A magnetic-labelled receptor that binds to annexin is added to sperm. Inside normal cells, phosphatidylserine molecules are located within the cell membrane towards the cytoplasm. Nevertheless, in those cells that initiate the apoptotic process phosphatidylserine instead faces the cell membrane outer side, binding to the annexin conjugate.
Previously, this procedure involved preparing a peripheral blood smear and manually counting each type of cell under a microscope, a process that typically required a half-hour. A Coulter counter played an important role in the development of the first cell sorter, and was involved in the early development of flow cytometry. Some flow ...
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