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A broadband “white light” source is used to illuminate the test and reference surfaces. A condenser lens collimates the light from the broadband light source. A beam splitter separates the light into reference and measurement beams. The reference beam is reflected by the reference mirror, while the measurement beam is reflected or scattered ...
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Optical coherence tomogram of a fingertip. It is possible to observe the sweat glands, having "corkscrew appearance" Interferometric reflectometry of biological tissue, especially of the human eye using short-coherence-length light (also referred to as partially-coherent, low-coherence, or broadband, broad-spectrum, or white light) was investigated in parallel by multiple groups worldwide ...
Watson interferometer (microscopy) White-light interferometer (see also Optical coherence tomography, White light interferometry, and Coherence Scanning Interferometry) White-light scatterplate interferometer (white-light) (microscopy) Young's double-slit interferometer; Zernike phase-contrast microscopy
Figure 1. The light path through a Michelson interferometer.The two light rays with a common source combine at the half-silvered mirror to reach the detector. They may either interfere constructively (strengthening in intensity) if their light waves arrive in phase, or interfere destructively (weakening in intensity) if they arrive out of phase, depending on the exact distances between the ...
Figure 3. White light interferometric microscope. White-light interferometry scanning (WLS) systems capture intensity data at a series of positions along the vertical axis, determining where the surface is located by using the shape of the white-light interferogram, the localized phase of the interferogram, or a combination of both shape and phase.
The technique encompasses but is not limited to instruments that use spectrally broadband, visible sources (white light) to achieve interference fringe localization. CSI uses either fringe localization alone or in combination with interference fringe phase, depending on the surface type, desired surface topography repeatability and software ...
The signal can be acquired with a camera in wide-field operation (a, b) or by point detection in confocal arrangement (c, d). Interferometric scattering microscopy (iSCAT) refers to a class of methods that detect and image a subwavelength object by interfering the light scattered by it with a