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In organic chemistry, Fehling's solution is a chemical reagent used to differentiate between water-soluble carbohydrate and ketone (>C=O) functional groups, and as a test for reducing sugars and non-reducing sugars, supplementary to the Tollens' reagent test. The test was developed by German chemist Hermann von Fehling in 1849. [1]
Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [2] For this reason it is essential to use multiple different tests to show all adulterants.
It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2] Tests that use this reagent are called Benedict's tests. A positive result of Benedict's test is indicated by a color change from clear blue to brick-red with a precipitate.
In analytical chemistry, a reagent is a compound or mixture used to detect the presence or absence of another substance, e.g. by a color change, or to measure the concentration of a substance, e.g. by colorimetry. Examples include Fehling's reagent, Millon's reagent, and Tollens' reagent.
The glucose solution is added to the solution containing indicator (dye indigo carmine) the color changes occur. This reaction is also known as chemical clock experiment because concentrations of the products and reactants changed over the specific period. [22] When the solution is shaken, oxygen dissolves in the solution and oxidizes indigo ...
Specifically, using Benedict's reagent and Fehling's solution the presence of the sugar is signaled by a color change from blue Cu(II) to reddish copper(I) oxide. [68] Schweizer's reagent and related complexes with ethylenediamine and other amines dissolve cellulose. [69]
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Aldonic acids are the products of the oxidation of aldoses by Benedict's or Fehling's reagents. [7] Copper ions react with an aldose to form a red precipitate, Cu 2 O. The reaction scheme of an aldose being oxidized by the copper ions in a Benedict's reagent solution. The R group provided is an example of a sugar backbone.