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The Gram stain results inform microbiologists about what types of agar plates should be used and what tests might be appropriate to identify the organism. [60] In some cases, no organisms are seen on the Gram stain despite the culture bottle showing indicators of growth or being reported as positive by automated instruments.
Gram-positive bacteria. Rod-shaped gram-positive Bacillus anthracis bacteria in a cerebrospinal fluid sample stand out from round white blood cells, which also accept the crystal violet stain. In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify ...
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
A Gram stain is performed to show Gram-positive cocci in chains. Then, the organism is cultured on blood agar. The rapid pyrrolidonyl arylamidase (PYR) test is commonly used, wherein a positive reaction confers a presumptive identification of group A beta-hemolytic streptococci if the appearance and clinical context is consistent.
Staphylococcus aureus is a gram-positive spherically shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe, meaning that it can grow without oxygen. [1]
Streptococcus mitior[1] Streptococcus mitis is a species of Gram-positive, mesophilic, alpha-hemolytic bacteria in the genus Streptococcus. These bacteria are facultative anaerobes, and made up of non-motile and non-sporing cocci (round cells) that are catalase negative. It is a commensal and commonly inhabits the human mouth, throat, and upper ...
Hauduroy et al. 1937. Clostridium perfringens (formerly known as C. welchii, or Bacillus welchii) is a Gram-positive, bacillus (rod-shaped), anaerobic, spore-forming pathogenic bacterium of the genus Clostridium. [1][2] C. perfringens is ever-present in nature and can be found as a normal component of decaying vegetation, marine sediment, the ...
Characteristics. Clostridium tetani is a rod-shaped, Gram-positive bacterium, typically up to 0.5 μm wide and 2.5 μm long. [1] It is motile by way of various flagella that surround its body. [1] C. tetani cannot grow in the presence of oxygen. [1] It grows best at temperatures ranging from 33 to 37 °C.