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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
High-performance thin-layer chromatography (HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds. [1] This TLC-based analytical technique enhances compound resolution for quantitative analysis.
Chiral chromatographic assay is the first step in any study pertaining to enantioselective synthesis or separation. This includes the use of techniques viz. gas chromatography (GC), high performance liquid chromatography (HPLC), chiral supercritical fluid chromatography (SFC), capillary electrophoresis (CE) [35] and thin-layer chromatography (TLC).
Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation technique of gas chromatography with the identification technique of mass spectrometry. GC-MS is the single most important analytical tool for the analysis of volatile and semi-volatile organic compounds in complex mixtures. [7]
However, the theoretical plate in packed beds, chromatography and other applications is defined as having a height. The empirical formula known as Van Winkle's Correlation can be used to predict the Murphree plate efficiency for distillation columns separating binary systems.
Chromatography separates dissolved substances by different interaction with (i.e., travel through) a material. High-performance liquid chromatography (HPLC) Thin-layer chromatography (TLC) Countercurrent chromatography (CCC) Droplet countercurrent chromatography (DCC) Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC)
Chiral thin-layer chromatography is a variant of liquid chromatography that is employed for the separation of enantiomers. It is necessary to use either It is necessary to use either a chiral stationary phase or
Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC). The setup has three components.