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Typical dot blot membrane. Darker dots indicate more protein. A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on ...
A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available. [1]
Protein:protein interaction studies; Colony blot, dot blot, Western blot and ELISA; Screening for positive expression clones; Immunocytochemistry and Immunohistochemistry; Protein localization and targeting studies; Because the Strep-tag is capable of isolating protein complexes, strategies for the study of protein-protein interactions can also ...
The far-western blot, or far-western blotting, is a molecular biological method based on the technique of western blot to detect protein-protein interaction in vitro. [1] [2] Whereas western blot uses an antibody probe to detect a protein of interest, far-western blot uses a non-antibody probe which can bind the protein of interest.
A southwestern blot is based on Southern blot and is used to identify and characterize DNA-binding proteins by their ability to bind to specific oligonucleotide probes. [2] The proteins are separated by gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting.
In a dot blot, macromolecules are applied directly to the matrix. Macromolecules can also be separated and transferred via gel electrophoresis. [1] One of the most common blotting matrices for protein analysis is nitrocellulose, which has a high affinity for proteins due to hydrophobic interactions. However, proteins with low molecular weight ...