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Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. The test is based on the fact that phenol red is excreted almost entirely in the urine.
OF-glucose deeps contain glucose as a carbohydrate, peptones, bromothymol blue indicator for Hugh-Leifson's OF medium or phenol red for King's OF medium, and 0.5% agar. To perform the OF-glucose test, two tubes of OF-glucose medium are inoculated with the test organism.
The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols , hydroxamic acids , oximes, and sulfinic acids give positive results as well. [ 1 ]
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
The doubly deprotonated (In 2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH) 3− form, and its pink color undergoes a rather slow fading reaction [ 6 ] and becomes completely colorless when pH is greater than 13.
The test employs the Trinder reagent, and is a colour change test resulting from the Trinder reaction. The Trinder reagent, named after P. Trinder of the Biochemistry Department of the Royal Infirmary in Sunderland (see the article listed in further reading), comprises an aminoantipyrine (such as 4-aminoantipyrine) and phenol (p-hydroxybenzene ...
The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Reagent test Alcohols: Forms Lucas test in alcohols is a test to differentiate between primary, secondary, and tertiary alcohols. Alkaloids: Forms Froehde Liebermann Mandelin Marquis Mayer's Mecke Simon's: Amines, and amino acids: Forms Folin's: Barbiturates: Class Dille–Koppanyi Zwikker: Benzodiazepines: Class Zimmermann: Phytocannabinoids ...