Search results
Results From The WOW.Com Content Network
Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens . [ 1 ]
For example, by mutating the F64L gene in jellyfish GFP, the protein is able to more efficiently fluoresce at 37 °C, an important attribute to have when growing cultures in a laboratory. [11] In addition to this, genetic engineering can produce a protein that emits light at a better wavelength or frequency. [ 11 ]
Immunofluorescence is a technique which uses the highly specific binding of an antibody to its antigen in order to label specific proteins or other molecules within the cell. A sample is treated with a primary antibody specific for the molecule of interest.
Antibody isotype(s) and location of antibody deposition in immunofluorescence studies using salt-split skin for autoimmune bullous conditions targeting the basement membrane zone of the human integumentary system Condition Antibody isotype(s) deposited Localization of antibody with use of salt-split skin Antiepilegrin cicatricial pemphigoid ...
A newer approach to immunoassays involves combining real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques. Called real-time immunoquantitative PCR (iqPCR) the label used in these assays is a DNA probe. [10] [11]
Because a real plant has a life of its own, we can care for it in a way that is not possible for the replica. I can help an acorn become an oak by planting it, but I can neither help a plastic ...
Example of a portable multiparametric fluorometer that uses the ratio between chlorophyll and flavonols to detect the nitrogen deficiency of plants. Because of the link between chlorophyll content and nitrogen content in leaves, chlorophyll fluorometers can be used to detect nitrogen deficiency in plants, by several methods.
Micrograph of paper autofluorescing under ultraviolet illumination. The individual fibres in this sample are around 10 μm in diameter.. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).