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Quenching is the basis for Förster resonance energy transfer (FRET) assays. [5] [6] [7] Quenching and dequenching upon interaction with a specific molecular biological target is the basis for activatable optical contrast agents for molecular imaging. [8] [9] Many dyes undergo self-quenching, which can decrease the brightness of protein-dye ...
The pulsed quenched-flow method, introduced by Alan Fersht and Ross Jakes, [9] eliminates the need for a long reaction tube. In this approach, the reaction is initiated as in a stopped-flow experiment, but quenching is performed using a third syringe, which delivers the quenching agent at a precise, pre-set time after initiation.
For diffusion-limited quenching (i.e., quenching in which the time for quencher particles to diffuse toward and collide with excited particles is the limiting factor, and almost all such collisions are effective), the quenching rate coefficient is given by = /, where is the ideal gas constant, is temperature in kelvins and is the viscosity of ...
is the fluorescence in the absence of halide F {\displaystyle F} is the fluorescence in the presence of halide K {\displaystyle K} is the Stern–Volmer quenching constant, which depends on the chloride concentration, [ C l − ] {\displaystyle [Cl^{-}]} . in a linear manner.
The Dexter energy transfer rate, , is indicated by the formula: = ′ [] where is the separation of the donor from the acceptor, is the sum of the Van der Waals radii of the donor and the acceptor, and ′ is the normalized spectral overlap integral, where normalized means that both emission intensity and extinction coefficient have been adjusted to unit area.
The efficiency of photochemical quenching (which is a proxy of the efficiency of PSII) can be estimated by comparing to the steady yield of fluorescence in the light and the yield of fluorescence in the absence of photosynthetic light . The efficiency of non-photochemical quenching is altered by various internal and external factors.
Here, I and I 0 denote the emission intensity with and without quenching agent present, k q the rate constant of the quenching process, τ 0 the excited-state lifetime in the absence of quenching agent and [Q] the concentration of quenching agent. Thus, if the excited-state lifetime of the photoredox catalyst is known from other experiments ...
Quenching (fluorescence), any process which decreases the fluorescence intensity of a given substance; Quenching (scrubber), a type of pollution scrubber; Magnet quench, a loss of superconductivity resulting in a rapid rise of resistance and temperature, such as in a superconducting magnet