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Experiment with Paper Chromatography. Chromatography is a technique used to separate a mixture or solution into its individual components. There are several different types of chromatography, including thin-layer, column, and paper chromatography.
WHAT IS CHROMATOGRAPHY? Chromatography is a technique used to separate mixtures. Information from a chromatography investigation can also be used to identify different substances. In chromatography, the mixture is passed through another substance, in this case, filter paper.
In the present experiment, we will use a method called chromatography. It is used quite widely for making small-scale separations and identifications. The method works because of the differences in the ways various components of a dissolved mixture interact with a fixed solid.
How Leaf Chromatography Works. Paper chromatography separates pigments in leaf cells on the basis of three criteria: Solubility; Molecule size; Polarity; Solubility is a measure of how well a pigment molecule dissolves in the solvent. In this project, the solvent is alcohol. Crushing the leaves breaks open cells so pigments interact with alcohol.
Chromatography is a way of separating the parts of a mixture so that you can see each component by itself. Paper chromatography has to be the best method for separating ink! When you dip the paper in water with a black marker drawn on it, the dried pigments from the marker ink dissolve.
Chromatography separates substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).
Performing a paper chromatography experiment involves several essential steps to ensure accurate results. The process begins with preparing samples for paper chromatography, then spotting the sample on the paper strip, and finally, developing the chromatogram.