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A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or ethical in living subjects. Tissues may be removed in many ways, including in part, as whole organs, or as larger organ systems. [citation needed] Examples of ex vivo specimen use include: [citation needed]
A live-cell microscope. Live-cell microscopes are generally inverted. To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy.
In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...
Ex vivo lung perfusion, EVLP, is a form of machine perfusion aimed at sustaining the active aerobic cellular metabolism of donor lungs outside the donor's body prior to lung transplantation. This medical preservation technique typically occurs within a specialised machine engineered to mimic the conditions of the natural circulatory system .
This is known as live-cell imaging. A few tools have been developed to identify and analyze single cells during live-cell imaging. [2] [3] [4] Time-lapse microscopy is the method that extends live-cell imaging from a single observation in time to the observation of cellular dynamics over long periods of time.
Molecular imaging is a field of medical imaging that focuses on imaging molecules of medical interest within living patients. This is in contrast to conventional methods for obtaining molecular information from preserved tissue samples, such as histology .
Intravital microscopy is a form of microscopy that allows observing biological processes in live animals at a high resolution that makes distinguishing between individual cells of a tissue possible. [1] In mammals, in some experimental settings a surgical implantation of an imaging window is performed prior to intravital microscopy. This allows ...
As fluorescence is a special case of luminescence, digital X-ray imaging is conceptually similar to digital gamma ray imaging (scintigraphy, SPECT, and PET) in that in both of these imaging mode families, the information conveyed by the variable attenuation of invisible electromagnetic radiation as it passes through tissues with various ...