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Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
[3]: 21–119 The spectrophotometer is used to measure colored compounds in the visible region of light (between 350 nm and 800 nm), [3]: 65 thus it can be used to find more information about the substance being studied. In biochemical experiments, a chemical and/or physical property is chosen and the procedure that is used is specific to that ...
A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
A colorimeter or a digital camera with a color filter array can, under certain conditions, be used as an alternative to a spectrophotometer. [7] [8] The illuminant and observer conditions should be specified when citing a measurement (e.g. D65/10°). [9] The quality of a colorimeter may be assessed using the means in CIE publication 179:2007. [10]
A colorimeter is a device used to test the concentration of a solution by measuring its absorbance of a specific wavelength of light. To use this device, different solutions must be made, and a control (usually a mixture of distilled water and another solution) is first filled into a cuvette and placed inside a colorimeter to calibrate the machine.
The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.