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Masson's trichrome is a three-colour staining procedure used in histology. The recipes emerged from Claude L. Pierre Masson 's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue .
The first staining protocol that was described as "trichrome" was Mallory's trichrome stain, which differentially stained erythrocytes to a red colour, muscle tissue to a red colour, and collagen to a blue colour. Some other trichrome staining protocols are the Masson's trichrome stain, Lillie's trichrome, and the Gömöri trichrome stain.
Masson's trichrome is (as the name implies) a three-colour staining protocol. The recipe has evolved from Masson's original technique for different specific applications, but all are well-suited to distinguish cells from surrounding connective tissue .
Lillie's trichrome is a combination of dyes used in histology. It is similar to Masson's trichrome stain , but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [ 1 ]
Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, [4] staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. The color intensifies with increasing number of double bonds in the molecule being stained. [5]
Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome, [5] as well as to reveal callose structures in plant tissues. [6] It can also be used in other connective tissue stains, such as Mallory's stain, [5] Gömöri trichrome stain, and Carstair's Method. [7] It is used in differential staining.
In Masson's trichrome, it is used as a counterstain to acid fuchsin. It is a component of Papanicolaou stains together with eosin Y and bismarck brown Y. [1] In pap smears, Light Green SF confers a blue staining for the cytoplasm of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. [3]
Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye used in histology for staining.It is easily soluble in water and slightly in ethanol.