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Many methods of performing Perls Prussian blue stain for iron have been published, [2] Drury and Wallington (1980) give a protocol that uses a mixture of 1 part 2% hydrochloric acid and 1 part 2% potassium ferrocyanide that is applied to the section for 20–30 minutes followed by a rinse in distilled water and application of a counterstain ...
A simple staining method for bacteria that is usually successful, even when the positive staining methods fail, is to use a negative stain. This can be achieved by smearing the sample onto the slide and then applying nigrosin (a black synthetic dye) or India ink (an aqueous suspension of carbon particles).
Micromass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. Alcian blue (/ ˈ æ l ʃ ə n /) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. [1]
Special staining techniques such as Albert's or Neisser's demonstrate the granules more clearly. Volutin granules are characteristically present in diphtheria bacilli. Their function is uncertain.
In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.
Moeller staining involves the use of a steamed dye reagent in order to increase the stainability of endospores. Carbol fuchsin is the primary stain used in this method. Endospores are stained red, while the counterstain methylene blue stains the vegetative bacteria blue.
These acids resist staining by ordinary methods such as a Gram stain. [9] It can also be used to stain a few other bacteria, such as Nocardia. The reagents used for Ziehl–Neelsen staining are carbol fuchsin, acid alcohol, and methylene blue. Acid-fast bacilli are bright red after staining. [citation needed]
Silver staining is used when more sensitive method for detection is needed, as classical Coomassie Brilliant Blue staining can usually detect a 50 ng protein band, Silver staining increases the sensitivity typically 10-100 fold more. This is based on the chemistry of photographic development.