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As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
The pupil function or aperture function describes how a light wave is affected upon transmission through an optical imaging system such as a camera, microscope, or the human eye. More specifically, it is a complex function of the position in the pupil [ 1 ] or aperture (often an iris ) that indicates the relative change in amplitude and phase ...
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
The ability of a lens to resolve detail is usually determined by the quality of the lens, but is ultimately limited by diffraction.Light coming from a point source in the object diffracts through the lens aperture such that it forms a diffraction pattern in the image, which has a central spot and surrounding bright rings, separated by dark nulls; this pattern is known as an Airy pattern, and ...
The sampling aperture can be a literal optical aperture, that is, a small opening in space, or it can be a time-domain aperture for sampling a signal waveform. For example, film grain is quantified as graininess via a measurement of film density fluctuations as seen through a 0.048 mm sampling aperture.
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light , and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
The optical transfer function of a well-focused (a), and an out-of-focus optical imaging system without aberrations (d). As the optical transfer function of these systems is real and non-negative, the optical transfer function is by definition equal to the modulation transfer function (MTF).
Due to Snell's law, the numerical aperture remains the same: NA = n 1 sin θ 1 = n 2 sin θ 2. In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light.