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Routine biochemical methods for identification of bacteria vary widely in their identification of this organism: the API 20NE system accurately identifies B. pseudomallei in 99% of cases, [26] as does the automated Vitek 1 system, but the automated Vitek 2 system only identifies 19% of isolates. [24]
Dilution has been used as a method to grow and identify bacteria since the 1870s, and as a method of testing the susceptibility of bacteria to antibiotics since 1929, also by Alexander Fleming. [25] The way of determining susceptibility changed from how turbid the solution was, to the pH (in 1942), to optical instruments. [25]
The analytical profile index, or API, is a classification system for bacteria based on biochemical tests. The system was developed to accelerate the speed of identifying clinically relevant bacteria. It can only be used to identify known species from an index. [1] The data obtained are phenotypic traits.
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...
Etest is a quantitative technique for determining the MIC of microoganisms. It is used for a range of Gram-negative and Gram-positive bacteria such as Pseudomonas, [2] [3] Staphylococcus, [4] and Enterococcus species, [5] as well as fastidious bacteria, such as Neisseria and Streptococcus pneumoniae. [1]
Historically, biochemical tests have been an important tool used to discriminate between Staphylococcus species. [20] [8] Tests used to identify S. pseudintermedius specifically include DNase, [13] hyaluronidase, [22] coagulase, catalase, and acetoin production tests, amongst others. [8]
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