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Zeocin is a trade name for a formulation of phleomycin D1, a glycopeptide antibiotic and one of the phleomycins from Streptomyces verticillus belonging to the bleomycin family of antibiotics. [1] It is a broad-spectrum antibiotic that is effective against most aerobic organisms including bacteria, filamentous fungi, yeast, plant, and animal cells.
G418 (geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1.It is produced by Micromonospora rhodorangea. [1] G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. [1]
Phleomycin D1 (zeocin) Phleomycin D2; Phleomycin E; References This page was last edited on 9 December 2023, at 11:58 (UTC). Text is available under the Creative ...
Invivogen was founded in 1997 in Toulouse, France. [1] The company is known for its mycoplasma detection and removal agents and its toll-like receptor product line. Although its first products focused on gene therapy, the company now produces tools for innate immunity research, immunology research, cancer research, RNA interference, cell culture, cloning and gene expression. [2]
β-Lactam antibiotics are indicated for the prevention and treatment of bacterial infections caused by susceptible organisms. At first, β-lactam antibiotics were mainly active only against gram-positive bacteria, yet the recent development of broad-spectrum β-lactam antibiotics active against various gram-negative organisms has increased their usefulness.
Blasticidin S is widely used in cell culture for selecting and maintaining genetically manipulated cells. Cells of interest express the blasticidin S resistance genes BSD or bsr, and can then survive blasticidin S being added to the culture media. [2]
The phleomycin/zeocin resistance gene Sh Ble is commonly used as a selection marker, [152] [155] and various transgenes have been successfully introduced and expressed in diatoms with stable transmissions through generations, [154] [155] or with the possibility to remove it.
In terms of resistance, for a specific clastogen known as "Zeocin", an amino acid residue known as XLF-L115D mutant is flawed in terms of being resistant thus the clastogen activity shows no amount of decreasing.