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The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Test Strips: The system consists of a plastic strip containing 20 small reaction tubes (microtubes), each containing different dehydrated substrates. Rehydration and Inoculation: To perform the test, the microtubes are rehydrated with a bacterial suspension. Each microtube is designed to detect specific enzymatic activities or carbohydrate ...
The aminopeptidase test analyzes bacteria for the production of the enzyme L-alanine-aminopeptidase, an enzyme found in many gram-negative bacteria. Adding L-Alanine-4-nitroanilide hydrochloride to a bacterial culture works as an indicator, changing to a yellow color in the presence of L-alanine-aminopeptidase.
Most of the samples used in both methods were cultivated aerobically in peptone-yeast extract-glucose-NaCl broth. [1] Several tests were performed to determine carbohydrate and physiological reactions, peptidoglycan type, the chemical makeup of the teichoic acids in the cell wall, and cytochrome pattern. [ 1 ]
Oxidative/fermentation glucose test (OF glucose test) is a biological technique. It was developed in 1953 by Hugh and Leifson to be utilized in microbiology to determine the way a microorganism metabolizes a carbohydrate such as glucose (dextrose). [ 1 ]
Voges–Proskauer / ˈ f oʊ ɡ ə s ˈ p r ɒ s k aʊ. ər / or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a glucose-phosphate broth that has been inoculated with bacteria. A cherry red color indicates a positive ...
Nitrogen fixation has been demonstrated in some strains of P. megaterium. [3] P. megaterium has been an important industrial organism for decades. It produces penicillin amidase used to make synthetic penicillin and several enzymes, such as amylases used in the baking industry and glucose dehydrogenase used in glucose blood tests. It also ...
In animals P. stuartii infections can cause neonatal diarrhea due to P. stuartii infection in dairy cows. In humans, P. stuartii can be isolated from urine (most common), stool, and blood, as well as from sputum, skin, and wound cultures. P. stuartii sepsis is primarily of urinary origin. It is the most common cause of purple urine bag syndrome ...