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The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.
To test for HIV, a series of blood screenings may be done, including one called the ELISA test. Read on to learn how these tests are done, what to expect during the tests, and what the results...
The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test...
For an HIV Western blot test, scientists work with prepared samples of HIV proteins and look to see if there are any antibodies in a person's blood that stick to them. Western blot HIV tests usually look for antibodies against the following HIV proteins: Proteins from the HIV envelope: gp41, and gp120/gp160.
The Western blot and ELISA tests are two blood antibody tests that may be used to detect HIV. In the past, the Western blot test was used to confirm the results of an ELISA test. However,...
Resources for all FDA-approved diagnostic HIV tests for use in moderate and high complexity laboratories, and provide the most recent guidance on HIV Laboratory Testing.
HIV-1 Western Blot Laboratory Tests. The HIV-1 Western blot has been largely replaced by more sensitive and specific HIV diagnostic tests. When used, the HIV-1 Western blot can detect human antibodies that react to HIV-1 proteins that originate from three HIV-1 gene regions: env (gp41, gp120/160), pol (p31, p51, p66), and gag (p15, p17, p24, p55) .
The Western blot - the most commonly used confirmatory test - is a highly specific immunoblot that allows for the visualization of antibodies to the structural polypeptides of HIV. Some laboratories may use a radioimmunoprecipitation assay as their confirmatory assay or as part of their HIV testing algorithm.
Reactive specimens from the initial test are subjected to an IgG-sensitive supplemental immunoassay capable of differentiating HIV-1 from HIV-2; this step replaces the HIV-1 Western blot.
Enzyme immunoassay (EIA) is the most widely used serologic test for detecting antibody to HIV-1. Serum samples that are repeatedly reactive in the EIA for HIV-1 antibody are then retested with a supplemental and more specific test, the most common of which is the Western blot (1-3).