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  2. Splice site mutation - Wikipedia

    en.wikipedia.org/wiki/Splice_site_mutation

    A splice site mutation is a genetic mutation that inserts, deletes or changes a number of nucleotides in the specific site at which splicing takes place during the processing of precursor messenger RNA into mature messenger RNA. Splice site consensus sequences that drive exon recognition are located at the very termini of introns. [1]

  3. Gene conversion - Wikipedia

    en.wikipedia.org/wiki/Gene_conversion

    Gene conversion is the process by which one DNA sequence replaces a homologous sequence such that the sequences become identical after the conversion. [1] Gene conversion can be either allelic, meaning that one allele of the same gene replaces another allele, or ectopic, meaning that one paralogous DNA sequence converts another.

  4. Human genome - Wikipedia

    en.wikipedia.org/wiki/Human_genome

    The human genome is a complete set of nucleic acid sequences for humans, encoded as the DNA within each of the 23 distinct chromosomes in the cell nucleus. A small DNA molecule is found within individual mitochondria.

  5. Primary transcript - Wikipedia

    en.wikipedia.org/wiki/Primary_transcript

    The process of gene expression itself is a source of endogenous DNA damages resulting from the susceptibility of single-stranded DNA to damage. [8] Other sources of DNA damage are conflicts of the primary transcription machinery with the DNA replication machinery, and the activity of certain enzymes such as topoisomerases and base excision ...

  6. Genetic engineering - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering

    This is an accepted version of this page This is the latest accepted revision, reviewed on 13 February 2025. Manipulation of an organism's genome For a non-technical introduction to the topic of genetics, see Introduction to genetics. For the song by Orchestral Manoeuvres in the Dark, see Genetic Engineering (song). For the Montreal hardcore band, see Genetic Control. Part of a series on ...

  7. RNA splicing - Wikipedia

    en.wikipedia.org/wiki/RNA_splicing

    RNA splicing is a process in molecular biology where a newly-made precursor messenger RNA (pre-mRNA) transcript is transformed into a mature messenger RNA ().It works by removing all the introns (non-coding regions of RNA) and splicing back together exons (coding regions).

  8. Overlap extension polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Overlap_extension...

    Besides the introduction of mutations, Overlap Extension PCR is widely used to assemble complex DNA sequences without the introduction of undesired nucleotides at any position. This is possible since OE-PCR relies on the utilization of complementary overhangs to guide the scarless splicing of custom DNA fragments in a desired order.

  9. Trans-splicing - Wikipedia

    en.wikipedia.org/wiki/Trans-splicing

    Trans-splicing is a special form of RNA processing where exons from two different primary RNA transcripts are joined end to end and ligated.It is usually found in eukaryotes and mediated by the spliceosome, although some bacteria and archaea also have "half-genes" for tRNAs.