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The term in situ in the medical context is part of a group of two-word Latin expressions, including in vitro, in vivo, and ex vivo. Similar to abbreviations, these terms support the concise transfer of essential information in medical communication. In situ is among the most widely used and versatile Latin terms in medical discourse in modern ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In-situ conservation is the on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species. [1] This process protects the inhabitants and ensures the sustainability of the environment and ecosystem.
In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA , [ 3 ] [ 4 ] [ 5 ] lncRNA [ 6 ] [ 7 ] [ 8 ] and miRNA in tissues and cells.
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
Examples of procedures that use oligonucleotides include DNA microarrays, Southern blots, ASO analysis, [3] fluorescent in situ hybridization (FISH), PCR, and the synthesis of artificial genes. Oligonucleotides are composed of 2'-deoxyribonucleotides (oligodeoxyribonucleotides), which can be modified at the backbone or on the 2' sugar position ...
Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue. [1] The historical precursor to spatial transcriptomics is in situ hybridization, [2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.
The in situ cyclization of proteins (INCYPRO) is a protein engineering technology that increases the durability of proteins and enzymes for biotechnological and biomedical applications. [ 1 ] [ 2 ] For such applications, it is essential that the used proteins maintain their structural integrity. [ 3 ]