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Space exploration utilizes in situ planetary research methods, conducting direct observational studies and data collection on celestial bodies, thereby avoiding the complexities inherent in sample-return missions. The humanities, notably archaeology, employ in situ methodologies to maintain contextual authenticity.
This list of research methods in biology is an index to articles ... Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
This technology is still in a developmental stage but, like other lab on a chip methods, it may lead to more portable diagnostic techniques. [28] [29] General process of fluorescent in situ hybridization (FISH) used for bacterial pathogen identification. First, an infected tissue sample is taken from the patient.
[3] [14] It also requires an extremely high sequencing depth of around 5 billion paired-end reads per sample to achieve the resolution of data described by Rao et al. [3] [14] [22] Several techniques that have adapted the concept of in situ Hi-C exist, including Sis Hi-C, OCEAN-C and in situ capture Hi-C. [3] Described below are two of the most ...
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
Figure 1: PLA starts with the binding of antibodies from different species to 2 proteins of interest, in this case protein * (star) and protein#. Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, extracellular vesicles and post translational modifications with high ...
Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue. [1] The historical precursor to spatial transcriptomics is in situ hybridization, [2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.