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In analytical chemistry, quantitative analysis is the determination of the absolute or relative abundance (often expressed as a concentration) of one, several or all particular substance(s) present in a sample. [1] It relates to the determination of percentage of constituents in any given sample. [2]
As an example, biological activity can be expressed quantitatively as the concentration of a substance required to give a certain biological response. Additionally, when physicochemical properties or structures are expressed by numbers, one can find a mathematical relationship, or quantitative structure-activity relationship, between the two.
A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
The ease of quantification is one of the features used to distinguish hard and soft sciences from each other. Scientists often consider hard sciences to be more scientific or rigorous, but this is disputed by social scientists who maintain that appropriate rigor includes the qualitative evaluation of the broader contexts of qualitative data.
For example: HCl + NaOH → NaCl + H 2 O. Acidimetry is the specialized analytical use of acid-base titration to determine the concentration of a basic (alkaline) substance using standard acid. This can be used for weak bases and strong bases. [8] An example of an acidimetric titration involving a strong base is as follows: Ba(OH) 2 + 2 H + → ...
For quantitative analysis, data is coded usually into measured and recorded as nominal or ordinal variables.. Questionnaire data can be pre-coded (process of assigning codes to expected answers on designed questionnaire), field-coded (process of assigning codes as soon as data is available, usually during fieldwork), post-coded (coding of open questions on completed questionnaires) or office ...
Elemental analysis is a process where a sample of some material (e.g., soil, waste or drinking water, bodily fluids, minerals, chemical compounds) is analyzed for its elemental and sometimes isotopic composition.
The concentration of a certain protein in a sample may be determined using spectrophotometric procedures. [5] The concentration of a protein can be determined by measuring the OD at 280 nm on a spectrophotometer, which can be used with a standard curve assay to quantify the presence of tryptophan, tyrosine, and phenylalanine. [6]